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Fig. 3 | Molecular Medicine

Fig. 3

From: Acid sphingomyelinase mediates ferroptosis induced by high glucose via autophagic degradation of GPX4 in type 2 diabetic osteoporosis

Fig. 3

Autophagy level and ASM/ceramide system expression were measured in hFob1.19 cells. Cells were treated with NG (17.5 mM) and HG (26.25 mM, 35 mM, 43.75 mM, respectively) for 48 h. A hFob1.19 cells expressing ptfLC3 were treated with different conditions, and fluorescent images were captured using LSCM. The number of autophagosomes represented by yellow puncta and autolysosomes represented by red puncta in merged images. Scale bar = 20 μm. B Western blot analysis of indicated proteins were analyzed. C Western blot analysis of LC3 II was carried out. D Ceramide levels were measured in hFob1.19 cells. All data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Molecular Medicine

ISSN: 1528-3658