Fig. 5

ASM decreases osteogenic potential by inducing ferroptosis in hFob1.19 cells. hFob1.19 cells were treated with NG (17.5 mM) or HG (35 mM) for 48 h in the presence or absence of ASM-shRNA. The cells were cultured with Fer-1 (5 µM) followed by HG for 48 h. Following the treatment, A cell survival was tested by CCK-8 assay. B Cell cytotoxicity was quantified using LDH content kit. C The proliferation of hFob1.19 cells was detected by EdU assay. Scale bar = 200 μm. D The osteogenic function of hFob1.19 cells was evaluated using an ALP staining kit after indicated treatments. E Mineralized extracellular matrix in hFob1.19cells was shown by alizarin red S staining. Scale bar = 200 μm. All data are presented as the mean ± SD of three independent experiments. **P < 0.01, ****P < 0.0001