Fig. 7

Contribution of ASM to the autophagic degradation of GPX4. hFob1.19 cells were treated with HG (35 mM) in the presence or absence of ASM-shRNA, HCQ (10 µM) and NAC (5 mM). Following the treatment, A Western blot analysis of GPX4 expression was carried out. Relative density of each protein bands were quantified, and the data are presented as the mean ± SD of three independent experiments. ****P < 0.0001. B Atg5 protein levels inhFob1.19 cells demonstrated by Western blotting. C hFob1.19 cells were treated with HG (35 mM) in the presence or absence of Atg5-shRNA. Western blot analysis of GPX4 was carried out. D Beclin 1 protein levels in hFob1.19 cells verified by Western blot analysis. E hFob1.19 cells were treated with HG (35 mM) in the presence or absence of Beclin 1-shRNA. Western blot analysis of GPX4 was carried out. F ASM-shRNA-transfected hFob1.19 cells were treated with HCQ (10 µM) for 12 h. Western blot analysis of GPX4 was carried out. All data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001