Fig. 11

Verification of the mechanism of serum Exos-mediated regulation of miR-382-3p and BDNF expression through MALAT1 in hippocampal neurons. A StarBase and Targetscan online prediction websites predicted targeting binding sites of MALAT1 with miR-382-3p and miR-382-3p with BDNF; B dual-luciferase experiments verified the targeting relationships between MALAT1 and miR-382-3p, and miR-382-3p and BDNF (P < 0.05 compared with the mimic-NC group); C RIP experiments verified the enrichment levels of MALAT1, miR-382-3p, and BDNF binding with AGO2 (P < 0.05 compared with the Anti-lgG group); D RT-qPCR detection of miR-382-3p inhibition efficiency (P < 0.05 compared with the inhibitor-NC group); E RT-qPCR detection of MALAT1 and miR-382-3p expression in each group of neurons (*P < 0.05 compared with the Exos + sh-NC + inhibitor-NC group, ^#P < 0.05 compared with the Exos + sh-MALAT1 + inhibitor-NC group); F Western blot detection of BDNF expression levels in each group of neurons; *P < 0.05 compared with the Exos + sh-NC + inhibitor-NC group, ^#P < 0.05 compared with the Exos + sh-MALAT1 + inhibitor-NC group; all cell experiments were repeated three times