Fig. 2

Effects of AE on protein expression and neuronal apoptosis in the hippocampus of T2DM mice. A Western blot detection of INSR, IRS-1, and IRS-2 protein expression in the hippocampus of mice in each group; B Western blot detection of PI3K, AKT, p-AKT, Ras, Erk1/2, and p-Erk1/2 protein expression in the hippocampus of mice in each group; C TUNEL staining was used to detect neuronal apoptosis in the hippocampus of mice in each group (×400); D BF-188 staining was used to detect changes in amyloid plaques and neurofibrillary tangles in the hippocampal region of mice in each group (×200, white arrows indicate amyloid plaques, red arrows indicate neurofibrillary tangles); n = 10, *P < 0.05 compared with blank group, ^#P < 0.05 compared with HF group