Fig. 5

Effects of AE-mediated serum Exos on proliferation and apoptosis of hippocampal neurons. A RT-qPCR detection of MALAT1 and miR-382-3p expression in neurons; B Western blot detection of INSR, IRS-1, and IRS-2 expression in hippocampal neurons; C CCK-8 detection of hippocampal neuron activity in each group; D EdU experiment was used to detect hippocampal neuron apoptosis in each group; E Flow cytometry was used to detect hippocampal neuron apoptosis in each group; F Western blot detection of PI3K, AKT, p-AKT, Ras, Erk1/2, and p-Erk1/2 expression in hippocampal neurons; G Western blot detection of Cleaved Caspase-3, Caspase-3, Bax, and BCL-2 expression in hippocampal neurons; H Western blot detection of SNAP-25 and VGluT-1 expression in hippocampal neurons; *P < 0.05 compared with the control group, ^#P < 0.05 compared with the HG + HF + Exos group, ^$P < 0.05 compared with the HG + HF + AE + Exos + DMSO group; all cell experiments were repeated three times