Fig. 6

Effect of AE-mediated serum-Exos on cognitive impairment in T2DM mice. A Water maze test was used to detect the escape latency time during the platform searching experiment, time spent in the target quadrant, and the time spent crossing the target platform in each group of mice; B Y-maze test was used to detect the percentage of spontaneous alteration in each group of mice; C changes in body weight in each group of mice; D ELISA detection of fasting blood glucose levels in each group of mice; E ELISA detection of fasting serum insulin levels in each group of mice; F Comparison of insulin resistance index of each group of mice; G Western blot detection of INSR, IRS-1, and IRS-2 protein expression in mouse hippocampus; H Western blot detection of PI3K, AKT, p-AKT, Ras, Erk1/2, and p-Erk1/2 expression in mouse hippocampus; I TUNEL staining was used to detect the apoptosis of brain hippocampal neurons in each group of mice (×400); J BF-188 staining was used to detect changes in senile plaques and neurofibrillary tangles in the brain hippocampus of each group of mice (×200, white arrows indicate senile plaques, red arrows indicate neurofibrillary tangles); n = 10, *P < 0.05 compared with the HF group, ^#P < 0.05 compared with the HF + Exos + DMSO group