Fig. 8

The effect of silencing MALAT1 on cognitive impairment in T2DM mice. A RT-qPCR detection of hippocampal MALAT1 expression in each group of mice; B Water maze test was used to detect the escape latency during the platform searching experiment, time spent in the goal quadrant, and time to cross the platform in each group of mice; C Y-maze test was used to detect spontaneous alternation percentage in each group of mice; D Changes in body weight of each group of mice; E ELISA detection of fasting blood glucose levels in each group of mice; F ELISA detection of fasting serum insulin levels in each group of mice; G Comparison of insulin resistance index in each group of mice; H Western blot detection of INSR, IRS-1, and IRS-2 expression in the hippocampus of each group of mice; I Western blot detection of PI3K, AKT, p-AKT, Ras, Erk1/2, and p-Erk1/2 expression in the hippocampus of each group of mice; J TUNEL staining was used to detect hippocampal neuronal apoptosis in each group of mice (×400); K BF-188 staining was used to detect changes in senile plaques and neurofibrillary tangling in the hippocampus of each group of mice (×200, white arrows indicate senile plaques, red arrows indicate neurofibrillary tangling); n = 10, * indicates P < 0.05 compared to the HF + Exos + sh-NC group