Fig. 9

The effect of overexpressing miR-382-3p on hippocampal neuronal apoptosis. A RT-qPCR detection of overexpression efficiency of miR-382-3p (P < 0.05 compared with the mimic-NC group); B RT-qPCR detection of hippocampal neuronal miR-382-3p expression in each group; C Western blot detection of INSR, IRS-1, and IRS-2 expression in hippocampal neurons; D CCK-8 detection of hippocampal neuron activity in each group; E EdU experiment was used to detect hippocampal neuronal apoptosis in each group; F flow cytometry was used to detect hippocampal neuronal apoptosis in each group; G Western blot detection of PI3K, AKT, p-AKT, Ras, Erk1/2, and p-Erk1/2 expression in hippocampal neurons; H Western blot detection of Cleaved Caspase-3, Caspase-3, Bax, and BCL-2 expression levels in hippocampal neurons; I Western blot detection of SNAP-25 and VGluT-1 expression in hippocampal neurons; *P < 0.05 compared with the HG + Exos + mimic-NC group; n = 10, *P < 0.05 compared with the HF + Exos + agomir-NC group; all cell experiments were repeated three times