Fig. 3

L-BAIBA protects the lung against ferroptosis induced by I/R injury

(A): The level of reactive oxygen species (ROS) in the lung was evaluated by dihydroethidium (DHE) staining (n = 5). Representative images are shown. The fluorescence intensity was analyzed by Image J (n = 5). (B): The concentrations of MDA in the lungs were measured (n = 5). (C): The SOD activity in the lungs was measured by commercially available kits (n = 5). (D): The ratio of GSH to GSSG was used to measure the oxidative stress level (n = 5). (E): Western blot was used to detect changes in the protein expression levels of GPX4 and SLC7A11 in the lung injured by I/R with or without L-BAIBA treatment (n = 5). Kolmogorov-Smirnov test was used for normality test. ANOVA was used for statistical analysis and Tamhane test (A, B, D, and E3) or LSD (C and E2) was used for post hoc analysis. The values are presented as mean ± SD (*P < 0.05 vs. Sham, #P < 0.05 vs. I/R only)