Fig. 4
L-BAIBA protects A549 cells from A/R injury by inhibiting ferroptosis
(A): Western blot was used to detect the protein expressions of GPX4 and SLC7A11 in A549 cells before or after A/R injury with or without L-BAIBA treatment (n = 4). (B and C): Effects of L-BAIBA on A549 cells injured by A/R were determined by measuring CCK8 activity and LDH release. A549 cells were treated with different concentrations of L-BAIBA (0.1–100 µM) (n = 8). (D): MDA concentration was to detect lipid peroxidation in A549 cells (n = 5). (E and F): The effects of the ferroptosis inducers RSL-3 and erastin on the activity of A/R-injured cells were determined by CCK8 activity (n = 8) and the release of LDH (n = 8). Kolmogorov-Smirnov test was used for normality test and Kruskal-Wallis test was used for statistical analysis. (G): The effect of ferroptosis inducers RSL-3 and erastin on lipid peroxidation after A/R injury was detected by measuring MDA concentrations (n = 5). (H): Images of Fe2+ probe staining. The experiment was repeated 5 times, and a representative image is shown. Average fluorescence intensity was analyzed by Image J. The values are presented as mean ± SD (*P < 0.05 vs. Control, #P < 0.05 vs. A/R only, &P < 0.05 vs. L-BAIBA + A/R). Kolmogorov-Smirnov test was used for normality test. ANOVA was used for statistical analysis and post hoc analysis was performed by using Tamhane test (A3, B, C, D, and F) or LSD (A2, G, and H)