Fig. 5

L-BAIBA inhibits ferroptosis through the Nrf-2 signaling pathway in A549 cells

(A): Western blot was used to detect the changes in the protein expressions of several anti-ferroptosis signaling proteins, such a P53, BAP1, PARP1, ATF3, and Nrf-2 (n = 3). (B): The protein levels of Nrf-2 in the nuclear extracts were analyzed by western blot lungs with or without I/R and with or without L-BAIBA treatment (n = 5). (C and D): The role of Nrf-2 signaling pathway on the protective effect of L-BAIBA was confirmed by blocking experiments; brusatol was used as the inhibitor of Nrf-2. The effects were determined by measuring CCK8 activity and LDH release (n = 8). (E): Western blot was used to detect changes in the protein expressions of GPX4 and SLC7A11 in A549 cells with or without interfering the expression of Nrf-2 (n = 4). The values are presented as mean ± SD (*P < 0.05 vs. Control or Sham, #P < 0.05 vs. A/R or I/R only, &P < 0.05 vs. L-BAIBA + A/R). Kolmogorov-Smirnov test was used for normality test. ANOVA was used for statistical analysis and post hoc analysis was performed by using LSD (A2, A5, A6, and E) or Tamhane test (A3, A4, B, C, and D)