Fig. 1

I/R injury occurs in a mouse model of intestinal I/R. (a) Representative HE staining images of intestinal mucosa after 15 min, 30 and 60 min of reperfusion (n = 8). Scale bars: 200 μm. (b) Chiu’s score of intestinal tissue injury after different reperfusion times (n = 8). (c) Quantitation of IL6, LDH, and TNF-α in serum after 15 min, 30 and 60 min of reperfusion (n = 8). (d) Representative Western blots of the TJ proteins ZO-1, Occludin, and SIRT3 after 15 min, 30 and 60 min of reperfusion (n = 8). (e) Quantitation of TJ proteins expressed after different reperfusion times (n = 8). The red arrow represents the Gruen-Hagen space. Data are presented as the mean ± SD. All experiments were repeated in triplicate. * P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Kruskal-Wallis H test (b), one-way ANOVA (c, e)