Fig. 2
Ferroptosis occurs in a mouse model of intestinal I/R. (a) Representative transmission electron microscopy images showing mitochondrial structure and morphology (n = 8). Scale bars: 500 nm. (b) Representative Western blots of the ferroptosis-associated proteins GPX4, FTH1 and ACSL4 after 15 min, 30 and 60 min of reperfusion (n = 8). (c) Quantitation of ferroptosis-associated proteins expressed after different reperfusion times (n = 8). (d) Total iron, Fe2+, Fe3+, MDA, total glutathione, GSH, GSSG, and GSH/GSSG levels were evaluated in reperfusion tissues (n = 8). The red arrows represent ruptured mitochondrial membranes. Data are presented as the mean ± SD. All experiments were repeated in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One-way ANOVA (c, d)