Fig. 3

 H/R increases the sensitivity of RSL3 to induce ferroptosis in Caco-2 cells. (a) Viability of cells treated with different concentrations of RSL3 for 24 h with or without H/R (n = 3). (b) Representative Western blots of SIRT3 treated with or without H/R and/or RSL3 (n = 3). (c) Quantitation of SIRT3 treated with or without H/R and/or RSL3 (n = 3). (d) Quantification of the MFI of the IF image (n = 3). (e) Representative IF image indicating LPO (green fluorescence) in cells treated with or without H/R and/or RSL3 (n = 3). Scale bars: 200 μm. Data are presented as the mean ± SD of three independent experiments in triplicates. **P < 0.01, ****P < 0.0001. Two-way ANOVA (c, d)