Fig. 4

Sirt3^−/−increases intestinal I/R injury and ferroptosis in a mouse I/R model. (a) Representative HE staining images of the intestinal mucosa of Sirt3^+/+ and Sirt3^−/− mice after I/R or without I/R (n = 8). Scale bars: 200 μm. (b). Chiu’s score of intestinal tissue injury in Sirt3^+/+ and Sirt3^−/− mice after I/R or without I/R (n = 8). (c) Quantification of FD4 and IFABP levels in the serum of Sirt3^+/+ and Sirt3^−/− mice after I/R or without I/R (n = 8). (d) Representative Western blots of ZO-1, Occludin, SIRT3, GPX4, FTH1, and ACSL4 levels from intestinal tissue of Sirt3^+/+ and Sirt3^−/− mice after I/R or without I/R (n = 8). (e) Quantification of these proteins from intestinal tissues of Sirt3^+/+ and Sirt3^−/− mice after I/R or without I/R (n = 8). Data are presented as the mean ± SD. All experiments were repeated in triplicate. *P < 0.05, **P < 0.01, ****P < 0.0001. Kruskal-Wallis H test (b), Two-way ANOVA (c, e)