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Fig. 5 | Molecular Medicine

Fig. 5

From: Resveratrol reduces ROS-induced ferroptosis by activating SIRT3 and compensating the GSH/GPX4 pathway

Fig. 5

SIRT3knockdown increases sensitivity to RSL3-induced ferroptosis. (a) Representative Western blots of SIRT3 expressed in shNC and shSIRT3-Caco-2 cells (n = 3). (b) Quantification of SIRT3 expression in shNC and shSIRT3-Caco-2 cells (n = 3). (c) Quantitation of cell viability in shNC and shSIRT3-Caco-2 cells treated with or without RSL3 (n = 3). (d) Representative LIVE/DEAD staining images showing cell viability in shNC and shSIRT3-Caco-2 cells treated with or without RSL3 (n = 3). Scale bars: 200 μm. (d) Quantification of oxidized BODIPY in shNC and shSIRT3-Caco-2 cells treated with or without RSL3 (n = 3). (f) Representative fluorescence images showing the levels of reduced BODIPY, oxidized BODIPY and Hoechst in shNC and shSIRT3 Caco-2 cells treated with or without RSL3 (n = 3). Scale bars: 200 μm. Data are presented as the mean ± SD of three independent experiments in triplicates. *P < 0.05, ***P < 0.001, ****P < 0.0001. Student’s t test (b), two-way ANOVA (c, d)

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Molecular Medicine

ISSN: 1528-3658