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Fig. 7 | Molecular Medicine

Fig. 7

From: Resveratrol reduces ROS-induced ferroptosis by activating SIRT3 and compensating the GSH/GPX4 pathway

Fig. 7

Resveratrol attenuates RSL3-induced ferroptosis dependent onSIRT3activation. (a) Viability of Caco-2 cells treated with resveratrol at concentrations of 0, 2.5, 5, 10, 20, and 40 µM for 24 h, 48 and 72 h (n = 3). (b) Representative Western blots of GPX4, FTH1 and ACSL4 levels in shNC-Caco-2 cells and shSIRT3-Caco-2 cells treated with resveratrol and/or RSL3 (n = 3). (c) Quantitation of GPX4, FTH1 and ACSL4 levels in shNC-Caco-2 cells and shSIRT3-Caco-2 cells treated with resveratrol and/or RSL3 (n = 3). (d) Representative IF image indicating SIRT3 in shNC-Caco-2 cells and shSIRT3-Caco-2 cells treated with resveratrol and/or RSL3 (n = 3). Scale bars: 200 μm. (e) Quantitation of the MFI of SIRT3 in shNC-Caco-2 cells and shSIRT3-Caco-2 cells treated with resveratrol and/or RSL3 (n = 3). The data are presented as the mean ± SD of three independent experiments in triplicates. *P < 0.05, **P < 0.01. Two-way ANOVA (c, d)

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Molecular Medicine

ISSN: 1528-3658