Fig. 2

CX3CL1 deficiency reduced ferroptosis in renal tissues with cisplatin-induced AKI. A–D RNA sequencing analysis. E–H Measurement of Fe²⁺, GSH/GSSG ratio, MDA, and SOD levels in kidney tissues. I Expression of ferritin heavy chain in kidney tissues, as measured using western blotting. J Representative images displaying the expression of 3-NT, 4-HNE, and GPX4 as determined by immunohistochemistry. Scale bar = 20 μm. K Western blotting analysis of GPX4 and XCT expression profiles. (AKI, acute kidney injury; GSH/GSSG, glutathione/oxidized glutathione; MDA, malondialdehyde; SOD, superoxide dismutase; 3-NT, 3-nitrotyrosine; 4-HNE, 4-Hydroxynonenal; GPX4, glutathione peroxidase 4; XCT, solute carrier family 7 member 11; P value was calculated by one-way analysis of variance and Tukey’s test. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the control group; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 compared to the Cis group)