Fig. 3

CX3CL1 knockdown inhibited cisplatin-induced ferroptosis in podocytes. A Expression of ferritin heavy chain in podocytes measured through western blotting. B–E The levels of Fe²⁺, GSH/GSSG ratio, MDA, and SOD were measured in podocytes. F Evaluation of XCT and GPX4 expression profiles in podocytes using western blotting. G Representative micrographs of podocytes stained with GPX4 and XCT. Scale bar = 10 μm. (GSH/GSSG, glutathione/oxidized glutathione; MDA, malondialdehyde; SOD, superoxide dismutase; XCT, solute carrier family 7 member 11; GPX4, glutathione peroxidase 4; p value was calculated by one-way analysis of variance and Tukey’s test. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the control group; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 vs. the Cis group)