Fig. 5

CX3CL1 deficiency attenuated kidney inflammation and macrophage infiltration in cisplatin-induced model mice. A Western blotting was conducted to assess the expression profiles of IL-6, TNF-α, and F4/80 in kidney tissues. B, C Levels of IL-6 and TNF-α in mouse sera. D Image representations of TNF-α and F4/80 expression by immunohistochemistry. Scale bar = 20 μm. E podocytes were transfected with siRNA-CX3CL1 as indicated and treated with cisplatin (10 μM) for 24 h. CX3CL1, TNF-α, and IL-6 expression profiles were then measured by western blotting. F, G Levels of IL-6 and TNF-α in podocyte cell supernatant. H, I Representative micrographs of CX3CL1 and TNF-α staining in podocytes. Scale bar = 10 μm. (IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; P value was calculated by one-way analysis of variance and Tukey’s test. *p < 0.05, **p < 0.01, and ***p < 0.001 vs the control group; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 vs the Cis group)