Fig. 1
RhoA-ROCK is up-regulated in human prostate tissues and increases the tension of human prostate strips. A qRT-PCR analysis demonstrated the mRNA expression level of RhoA, ROCK1 and ROCK2 in normal prostate tissue (n = 16) and BPH tissue (n = 16). B Immunoblot assay revealed the protein expression level of RhoA, ROCK1 and ROCK2 in normal prostate tissue and BPH tissue. C Immunofluorescence staining of RhoA for normal human prostate and BPH prostate. DAPI (blue) indicated nuclear staining and Cy3-immunofluorescence (red) indicated RhoA protein staining. D Immunohistochemical staining of RhoA for normal human prostate and BPH prostate. E Representative force tracings of prostate strips from normal human and BPH patients. After 15-min incubation with 10 μM Y-27632, strips were contracted with 10−5M PE and the inhibition of Y-27632 was evaluated. The summary graph of inhibition effect of Y-27632 on PE-mediated contraction of human prostate strips (n = 4 different humans for each group). The force for first response to PE was taken as 100% while the inhibition effect of Y-27632 was evaluated as a percentage of this response. Data were expressed as mean ± SD. **p < 0.01, ***p < 0.001