Fig. 2From: Mechanism of RhoA regulating benign prostatic hyperplasia: RhoA-ROCK-β-catenin signaling axis and static & dynamic dual rolesRhoA promotes the proliferation of WPMY-1 and BPH-1 cells. A Knockdown efficiency of RhoA at the mRNA levels with two different siRNA sequences in WPMY-1 and BPH-1 cells. B Overexpression efficiency of RhoA at the mRNA levels in WPMY-1 and BPH-1 cells. C Immunoblot assay of RhoA, ROCK1 and ROCK2 after RhoA knockdown. D Immunoblot assay of RhoA, ROCK1 and ROCK2 after RhoA overexpression. E The cell proliferation of WPMY-1 and BPH-1 cells measured by CCK-8 assay at different time points after RhoA knockdown. F The cell proliferation of WPMY-1 and BPH-1 cells measured by CCK-8 assay at different time points after RhoA overexpression. G The Ki-67 staining of WPMY-1 and BPH-1 cells after RhoA knockdown. Data were expressed as mean ± SD. **p < 0.01, ***p < 0.001Back to article page