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Fig. 4 | Molecular Medicine

Fig. 4

From: Mechanism of RhoA regulating benign prostatic hyperplasia: RhoA-ROCK-β-catenin signaling axis and static & dynamic dual roles

Fig. 4

RhoA promotes the fibrosis, EMT, cell contration and non-muscle myosin of WPMY-1 and BPH-1 cells. A Immunoblot assay of proteins in relation to cell fibrosis (α-SMA and collagen I) and EMT (E-Cad, N-Cad and Vimentin) in WPMY-1 and BPH-1 cells after RhoA knockdown. B Immunoblot assay of proteins in relation to cell fibrosis and EMT in WPMY-1 and BPH-1 cells after RhoA overexpression. C The contraction of WPMY-1 cells detected by collagen gel contraction assay at different time points after RhoA knockdown. D The contraction of WPMY-1 cells detected by collagen gel contraction assay at different time points after RhoA overexpression. E Immunoblot assay of proteins in relation to non-muscle myosin (NMMHC-A and NMMHC-B) in WPMY-1 and BPH-1 cells after RhoA knockdown. F Immunoblot assay of proteins in relation to non-muscle myosin in WPMY-1 and BPH-1 cells after RhoA overexpression. G The actin cytoskeleton detected by cell phalloidine staining in WPMY-1 and BPH-1 cells after RhoA knockdown. DAPI (blue) indicated nuclear staining, Phalloidine staining (red) indicated F-actin staining. Data were expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

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Molecular Medicine

ISSN: 1528-3658