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Fig. 6 | Molecular Medicine

Fig. 6

From: Mechanism of RhoA regulating benign prostatic hyperplasia: RhoA-ROCK-β-catenin signaling axis and static & dynamic dual roles

Fig. 6

Verification of co-transfection system and β-catenin nuclear translocation analysis in WPMY-1 and BPH-1 cells. A Overexpression efficiency of β-catenin at mRNA levels in BPH-1 and WPMY-1 cells. B Overexpression efficiency of β-catenin at protein levels in BPH-1 and WPMY-1 cells. C Immunoblot assay of RhoA, ROCK1 and ROCK2 in WPMY-1 and BPH-1 cells after co-transfection. D Immunoblot assay of Wnt/β-catenin downstream proteins (β-catenin, C-MYC, Survivin, Snail and CyclinD1) in WPMY-1 and BPH-1 cells after co-transfection. E Immunoblot assay of nucleus-proteins and cytoplasm-proteins (β-catenin) in WPMY-1 and BPH-1 cells after co-transfection. Data were expressed as mean ± SD. ns means no significant difference, *p < 0.05, ***p < 0.001

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Molecular Medicine

ISSN: 1528-3658