Fig. 7

RhoA-ROCK promotes cell proliferation, fibrosis and EMT, inhibits cell apoptosis of WPMY-1 and BPH-1 cells by regulating β-catenin. A The cell proliferation of WPMY-1 and BPH-1 cells measured by CCK-8 assay at different time points after co-transfection. B Flow cytometry analysis of the cell apoptosis in WPMY-1 and BPH-1 cells after co-transfection. C Immunoblot assay of proteins in relation to cell apoptosis (BAX and Bcl-2) in WPMY-1 and BPH-1 cells after co-transfection. D Immunoblot assay of proteins in relation to fibrosis (α-SMA and collagen I) and EMT (E-Cad, N-Cad and Vimentin) in WPMY-1 and BPH-1 cells after co-transfection. E The contraction of WPMY-1 cells detected by collagen gel contraction assay at different time points after co-transfection. F Immunoblot assay of proteins in relation to non-muscle myosin (NMMHC-A and NMMHC-B) in WPMY-1 and BPH-1 cells after co-transfection. G The contraction of WPMY-1 cells detected by collagen gel contraction assay at different time points after 10 μM Y-27632 treatment. Data were expressed as mean ± SD. ns means no significant difference, * p < 0.05, **p < 0.01, ***p < 0.001