Fig. 1

Genetic deficiency of TAOK3 has no effect on body weight or composition, food intake, or locomotor activity in high-fat diet-fed mice; however, total energy expenditure is lower in Taok3^–/– vs. wild-type mice. A Body weight curves. B Total, lean, and fat body mass measured by BCA after 18 weeks of high-fat feeding. C Weight of adipose tissue depots and liver. D, E Accumulated food consumption per day (D) and locomotor activity (E) after 15 and 14 weeks of high-fat diet challenge, respectively. F, G Total energy expenditure per hour (F) as well as during the light and dark phases of the measurements (G) determined at 21.0 °C and 29.5 °C after 15 weeks of high-fat diet consumption. Data are mean ± SEM from 8 to 9 mice per group. KO, knockout; TEE, total energy expenditure; Temp, temperature; WT, wild-type. Statistical significance between the groups was evaluated using the unpaired 2-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001