Fig. 2

Genetic ablation of TAOK3 does not affect glucose or insulin homeostasis in high-fat diet-fed mice. A, B Fasting circulating levels of glucose (A) and insulin (B). C HOMA-IR calculated using the equation [fasting glucose (mg/dl) × fasting insulin (ng/ml)]/405. D, E Intraperitoneal GTT (D) and ITT (E) performed after 16 and 17 weeks of high-fat feeding, respectively. The area under the glucose curve in each test. F Plasma levels of insulin during the GTT. G Insulin-stimulated glucose uptake in individual tissues quantified by in vivo ¹⁴C-2DG uptake assay after 18 weeks of high-fat diet consumption. Data are mean ± SEM from 6 to 7 (G) or 13 to 15 (A–F) mice per group. AUC, area under the curve; Edl, extensor digitorum longus; Gas, gastrocnemius muscle; KO, knockout; Quad, quadriceps muscle; Sol, soleus muscle; WT, wild-type. Statistical significance between the groups was evaluated using the unpaired 2-tailed Student’s t-test