Fig. 3

Depletion of TAOK3 does not protect mice against high-fat diet-induced liver steatosis, inflammation, or fibrosis. A Representative liver sections stained with Bodipy 493/503 (green), MitoTracker Red (red), or DHE (red), or processed for immunofluorescence with anti-cytochrome c (green), anti-CHOP (red), anti-F4/80 (green), anti-Gr1 (Ly6C) (red), or anti-collagen IV (red) antibodies; nuclei stained with DAPI (blue). The scale bars represent 25 µm. Quantification of the staining. B Representative liver sections stained with H&E or Picrosirius Red. The scale bars represent 100 µm. C Relative mRNA expression of selected genes controlling inflammation and fibrosis assessed by qRT-PCR in the liver. D Hepatic glycogen content. E Liver lysates analyzed by Western blot using anti-total OXPHOS antibody cocktail or antibodies specific for TAOK3. Protein levels analyzed by densitometry; representative Western blots are shown with vinculin used as a loading control. Data are mean ± SEM from 8 to 9 mice per group. HFD, high-fat diet; KO, knockout; WT, wild-type. Statistical significance between the groups was evaluated using the unpaired 2-tailed Student’s t-test