Fig. 4

TAOK3 deficiency has no effect on adipose tissue function in high-fat diet-fed mice. A Representative eWAT sections stained with H&E. The scale bars represent 100 µm. B, C Average adipocyte size (B) and adipocyte size distribution with values representing relative proportion of adipocytes in the given diameter class (C) in the eWAT. D Relative mRNA expression of adipokines and selected genes controlling lipid metabolism, oxidative/ER stress, and inflammation assessed by qRT-PCR in the eWAT. E BAT lysates analyzed by Western blot using antibodies specific for TH, UCP1, or TAOK3, or anti-total OXPHOS antibody cocktail. Protein levels analyzed by densitometry; representative Western blots are shown with vinculin used as a loading control. Data are mean ± SEM from 8 to 9 mice per group. HFD, high-fat diet; KO, knockout; WT, wild-type. Statistical significance between the groups was evaluated using the unpaired 2-tailed Student’s t-test