Fig. 5

Depletion of TAOK3 in high-fat diet-fed mice does not affect kidney or skeletal muscle lipotoxicity. A, B Assessment of TAG content (A) and the ratio of GSH/GSSG (B) in the kidney lysates. C Measurement of urinary albumin, creatinine, and albumin to creatinine ratio. D Representative gastrocnemius muscle sections stained with Nile Red (red); nuclei stained with DAPI (blue). The scale bars represent 10 µm. Quantification of the staining. E Representative gastrocnemius muscle sections stained for NDH, SDH, or COX activities. The scale bars represent 25 µm. F Glycogen content in the gastrocnemius muscle. G Relative mRNA expression of selected genes controlling lipid and glucose metabolism assessed by qRT-PCR in the gastrocnemius muscle. Data are mean ± SEM from 8 to 9 (A, B and D–G) or 11 (C) mice per group. KO, knockout; WT, wild-type. Statistical significance between the groups was evaluated using the unpaired 2-tailed Student’s t-test