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Fig. 9 | Molecular Medicine

Fig. 9

From: Leveraging a disulfidptosis-related signature to predict the prognosis and immunotherapy effectiveness of cutaneous melanoma based on machine learning

Fig. 9

Validates the role of the key gene HLA-DQA1 in melanoma cell lines in vitro. A Representative IHC images of HLA-DQA1 protein expression in CM and benign nevus tissues. B Knockdown of HLA-DQA significantly reduced its expression in A-375 and A-875 cell lines (**P < 0.01, ****P < 0.0001). C After HLA-DQA1 knockdown in A-375 and A-875 cell lines, the increase of cell proliferation was markedly enhanced (***P < 0.01, ****P < 0.0001). D Clonogenic assays showed a significant increase in the ability of A-375 and A-875 cell lines to form colonies after HLA-DQA1 knockdown (***P < 0.001). E The si-NC group in the wound healing experiment of A-375 and A-875 cell lines showed weaker migration ability than the si-HLA-DQA1 group (**P < 0.01, ***P < 0.001). F The invasion ability of A-375 and A-875 cell lines significantly increased after HLA-DQA1 knockdown (*P < 0.5, **P < 0.01)

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Molecular Medicine

ISSN: 1528-3658