Fig. 2

Abnormal CRTC2 were expressed in kidney of DKD mice. C57BL/6J mice were intraperitoneally injected with STZ (30 mg/Kg) or PBS control. The STZ mice were fed with high fat diet and control mice were fed with normal diet. A The mice were weighed (g) every 2 weeks. B OGTT and ITT were performed on the mice one week before death (n = 6–9). C 24 h urinary protein were measured after 10 weeks of treatment. Serum Fasting blood glucose (FBG), blood urea nitrogen (BUN) and serum creatinine (SCr) level (n = 6–9). D H&E staining in kidney tissue sections from mice. E CRTC2 were detected by real-time PCR in the kidney of mice (n = 6–9). F Immunohistochemistry analysis of CRTC2 in kidney tissue sections from mice and quantitative analysis were conducted by Image J software. G CRTC2 was detected by Western blot in the kidney of mice. The data were presented as means ± SD. The statistical significance was relative to the STZ group *p < 0.05, **p < 0.01