Fig. 5

CRTC2 promoted EMT through CREB-Smad2/3 signaling pathway. HK-2 cells were transfected with or without control vector, as well as CRTC2 overexpression plasmid or SiRNA Smad2/3 for 48 h. And cells were treated with TGF-β1 (8 ng/ml, 48 h). A α-SMA promoter activity was detected using dual luciferase reporters in triplicate. B–E Smad3, CRTC2, E-cadherin and α-SMA gene expression was analyzed by real-time PCR. F 293T cells were transfected with overexpression plasmid of CRTC2 (CRTC2-Flag) for 48 h, immunoblots of co-immunoprecipitation assay showing binding of CBP and CREB in cells. All experiments was assayed in triplicate, **p < 0.01 compared with Control group. ^##p < 0.01 compared with TGF-β1 group. ^$$p < 0.01 compared with CRTC2 overexpression plasmid group