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Fig. 1 | Molecular Medicine

Fig. 1

From: Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer

Fig. 1

CRC cell-derived exosomes promote angiogenesis of HUVECs. (A) TEM analysis of SW480 or HCT116 cell-derived exosomes. (B) NTA of SW480 or HCT116 cell-derived exosomes. (C) Exosome markers CD63, TSG101 and CD9 were detected by western blot. (D) The uptake of PKH26-labeled exosomes was examined by confocal microscopy. Red: PKH26; Blue: DAPI. Scale bar, 20 μm. (E) The circCOL1A1 level in HUVECs was determined by qRT-PCR. (F) Cell proliferation was monitored by CCK-8 assay. (G) Cell migration was assessed by wound healing assay. (H) The in vitro angiogenesis was detected by tube formation assay. (I) The protein levels of VEGFR and Smad1/2/3/4/6/7 were detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001

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Molecular Medicine

ISSN: 1528-3658