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Fig. 2 | Molecular Medicine

Fig. 2

From: Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer

Fig. 2

Exosomal circCOL1A1 promotes angiogenesis of HUVECs. SW480 and HCT116 cells were transfected with sh-NC, sh-circCOL1A1, OE-NC and OE-circCOL1A1. HUVECs were treated with the designated exosomes. The circCOL1A1 level in SW480 and HCT116 cells (A) or CRC cell-derived exosomes (B) was determined by qRT-PCR. (C) The circCOL1A1 level in HUVECs was determined by qRT-PCR. (D) Cell proliferation was monitored by CCK-8 assay. (E-F) Cell migration was assessed by wound healing assay. (G-H) The in vitro angiogenesis was detected by tube formation assay. (I) The protein levels of VEGFR and p-Smad2, Smad2, p-Smad3, and Smad3 were detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001

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Molecular Medicine

ISSN: 1528-3658