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Fig. 3 | Molecular Medicine

Fig. 3

From: Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer

Fig. 3

CircCOL1A1 directly binds to EIF4A3 protein in HUVECs. (A) Bioinformatics analysis of circCOL1A1-binding proteins by Circular RNA Interactome. (B-D) Data analyses on EIF4A3 expression based on TCGA data from UALCAN database. (E) The mRNA level of EIF4A3 in CRC tissues was determined by qRT-PCR. (F) The immunoreactivity of EIF4A3 in CRC tissues was detected by IHC. Scale bar, 100 μm. (G) The association between circCOL1A1 and EIF4A3 was detected by RNA pull-down assay. Anti-sense circCOL1A1 probe served as a negative control. (H) The co-localization of circCOL1A1 and EIF4A3 protein was assessed by RNA FISH and IF staining. Green, circCOL1A1; Red, EIF4A3; Blue, DAPI. Scale bar, 20 μm. (I) The association between circCOL1A1 and EIF4A3 was detected by RIP assay. Normal IgG served as a negative control. (J) The protein level of EIF4A3 in HUVECs was detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001

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Molecular Medicine

ISSN: 1528-3658