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Fig. 4 | Molecular Medicine

Fig. 4

From: Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer

Fig. 4

CircCOL1A1 promotes angiogenesis of HUVECs via recruiting EIF4A3. HUVECs were transfected with sh-NC, sh-circCOL1A1, sh-circCOL1A1 + pcDNA3.1-NC and sh-circCOL1A1 + pcDNA3.1-EIF4A3. (A) The circCOL1A1 level in HUVECs was determined by qRT-PCR. (B) The protein level of EIF4A3 in HUVECs was detected by western blot. (C) Cell proliferation was monitored by CCK-8 assay. (D) Cell migration was assessed by wound healing assay. (E) The in vitro angiogenesis was detected by tube formation assay. (F) The protein levels of VEGFR and p-Smad2, Smad2, p-Smad3, and Smad3 were detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001

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Molecular Medicine

ISSN: 1528-3658