Fig. 8

Cav-1 coordinates the interaction between VEGFR2 and VE-Cadherin at the plasma membrane. VEGFR2/VE-Cadherin PLA was performed in HUVECs transfected with control and Cav-1 siRNA. Immunostaining was performed using an anti-P120 antibody to visualize the plasma membrane (A and B). Representative images and high-magnification insets are shown to visualize the PLA products at the plasma membrane. Scale bar, 20 µm. A Quantification of the number of VEGFR2/VE-Cadherin complexes at the plasma membrane. B The data were obtained from three independent transfections. n = 40. The content of Cav-1 in cell batches used for the PLA was assessed by immunoblotting (C). **p < 0.01