Fig. 3
From: Loss of Krüppel-like factor-10 facilitates the development of chemical-induced liver cancer in mice
Induction of hepatocellular carcinoma (HCC) by diethylnitrosamine (DEN) treatment in KLF10 wild-type (WT) and knockout (KO) mice. A Two-week-old KLF10 KO (n = 9) and WT (n = 8) mice received intraperitoneal DEN injections weekly for 8 weeks. DEN-injected mice were sacrificed at the age of 24 weeks. Liver specimens were stained with hematoxylin and eosin (H&E) and evaluated for HCC development. Representative images of HCC (arrow) are shown. The boxed part of HCC in the low-magnification image (6 ×) is magnified (200 ×) and presented. B The HCC burden (size in mm × tumor number) was evaluated under microscopy after H&E staining in KLF10 WT (n = 8) and KO (n = 9) mice. (C Assessment of liver fibrosis by Colα1 mRNA expression after DEN treatment in KLF10 WT (n = 8) and KO (n = 9) mice within liver tumor sites. Age-matched WT (n = 8) or KO (n = 8) mice with no treatment served as the controls. *P < 0.005 compared with SD-fed WT or KO mice