Fig. 5
From: Loss of Krüppel-like factor-10 facilitates the development of chemical-induced liver cancer in mice
Invasion of Hep3B and LX2 cells. A Invasion of Hep3B and LX2 cells was determined using the Transwell assay. Transient KLF10 deletion was induced using siRNA. The number of invasive cells was counted in five random fields under a microscope at 200 × magnification and is shown as the mean ± standard deviation. Representative images from the experiment are shown. B Invasion of Hep3B cells was assessed after co-culture with LX2 cells with KLF10 either deleted or preserved. Hep3B and LX2 cells were cultured using hanging cell culture inserts (1-µm pore size, Falcon) to separate cell populations. Hep3B cells were seeded in the insert (3 × 103 cells/cm2) and allowed to attach overnight in Dulbecco’s modified Eagle medium with 10% fetal bovine serum. LX2 cells (3 × 105 cells/cm2) were seeded on the upper part of the filter of the Transwell chamber system, and siRNA transfection (KLF10 or control) was performed. The plate with LX2 cells was placed below the culture insert with Hep3B cells. The number of invasive cells was counted in five fields under a microscope at 200 × magnification and is shown as the mean ± standard deviation. Representative images from the experiment are shown. Scale bar = 50 µm