Fig. 1

Liver tissues of old mice exhibit decreased expression of IGF2 and aging liver microenvironment. A Western blotting analysis for the protein expression of IGF2 and key senescence-associated genes P53, P21 and P16 in the liver from mice of different ages. The quantitative data are presented. mRNA levels of IGF2 (B), senescence-associated genes P53, P21 and P16 (C), and senescence-associated secretory phenotype genes IL-6, IL-1β, TNF-α and NF-κB1 (D), as determined by RT‒qPCR analysis. n = 5 per group. Data are shown as means ± SEM. One-way ANOVA was used for comparison among multiple groups. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001