Fig. 2

Conditional knockout of IGF2 in hepatocyte accelerated d-gal-induced liver aging. The expression levels of IGF2 were examined by Western blotting (A) and RT‒qPCR (B). Liver function evaluated by ALT (C) and AST (D). E Western blotting analysis for the protein level of senescence-associated genes P53, P21 and P16 in the liver tissues. The quantitative data are presented. F Relative mRNA expression levels of senescence-associated secretory phenotype genes IL-6, IL-1β, TNF-α and NF-κB1 in the liver tissues, as measured by RT‒qPCR. G SA-β-gal staining of liver sections. β-gal staining area analysis was shown. Scale bar, 100 um. H H&E staining of liver sections. Scale bar, 50 um. I Immunofluorescent staining of F4/80 and DAPI on liver sections. Fluorescence intensity analysis was shown. Scale bar, 50 um. A–F n = 5–7 per group, G–I n = 3 per group. All values are shown as means ± SEM. Dots represent individual level data. One-way ANOVA was used for comparison among multiple groups. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001