Fig. 3

IGF2 deficiency regulated the transcription of senescence, inflammation and mitochondrial function. RNA-Seq was performed in the liver tissues of IGF2^f/f + d-gal and IGF2^f/fCre + d-gal mice (n = 3 per group). A Volcano plot showing DEGs and labeling top 20 DEGs. The red and blue dots indicated upregulated and downregulated genes, respectively. GO analysis of the upregulated DEGs (B) and downregulated DEGs (C). A heatmap showed genes associated senescence and inflammatory (D), mitochondrial oxidative phosphorylation and ATP metabolic process (E). GSEA and pathway enrichment analysis of pathways related to mitochondrial gene expression (F), oxidative phosphorylation (G), and ATP metabolic process (H). I Western blotting analysis for the protein levels of AKT, p-AKT, ERK1/2, p-ERK1/2, p38 and p-p38 (n = 5–7 per group). The quantitative data are presented. Data are shown as means ± SEM. One-way ANOVA was used for comparison among multiple groups. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001