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Fig. 3 | Molecular Medicine

Fig. 3

From: Downregulation of HMGB1 carried by macrophage-derived extracellular vesicles delays atherosclerotic plaque formation through Caspase-11-dependent macrophage pyroptosis

Fig. 3

Preparation and identification of macrophage-EV/siHMGB1. A The morphology of macrophage-EV and macrophage-EV/siHMGB1 observed by TEM (100 nm). B Diameter size of macrophage-EV and macrophage-EV/siHMGB1 measured by NTA. C CD63, CD9, and Calnexin content on EV surface detected by Western blot analysis. D Uptake of macrophage-EV and macrophage-EV/siHMGB1 in RAW264.7 cells observed by laser confocal microscope (400 × , 25 μm). The PKH67-labeled macrophage-EV and macrophage-EV/siHMGB1 showed green fluorescence. E Uptake of siHMGB1 by RAW264.7 cells analyzed by laser confocal microscope (400 × , 25 μm). The cy3-labeled siHMGB1 shows red fluorescence. F HMGB1 expression detected by RT-qPCR and Western blot after RAW264.7 cells were treated with macrophage-EV and macrophage-EV/siHMGB1. *Iindicates p < 0.05. Data between the two groups are compared using an independent sample t-test. Data among multiple groups are compared using one-way ANOVA, followed by Tuckey's post hoc test

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Molecular Medicine

ISSN: 1528-3658