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Fig. 6 | Molecular Medicine

Fig. 6

From: Downregulation of HMGB1 carried by macrophage-derived extracellular vesicles delays atherosclerotic plaque formation through Caspase-11-dependent macrophage pyroptosis

Fig. 6

Macrophage-EV/siHMGB1 inhibits Caspase-11-dependent macrophage pyroptosis to prevent atherosclerotic plaque formation. A Western blot analysis detected the Activation of Caspase-11 in macrophages following ox-LDL, LPS, or macrophage-EV/siHMGB1 treatment. B HMGB1 Expression in the cell culture supernatants following ox-LDL, LPS, or macrophage-EV/siHMGB1 treatment measured by ELISA. C Expression of pyroptosis-related inflammatory factors IL-1β and IL-18 in cell culture medium supernatants following ox-LDL, LPS, or macrophage-EV/siHMGB1 treatment determined by ELISA. D Expression of the pyroptosis-related proteins FL-GSDMD and N-GSDMD in cells following ox-LDL, LPS, or macrophage-EV/siHMGB1 treatment measured by Western blot analysis. E Pyroptosis in the aortic root of AS mice following macrophage-EV/siHMGB1 treatment studied by IF. *Indicates p < 0.05. n = 6. Data among multiple groups are compared using one-way ANOVA, followed by Tuckey’s post hoc test

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Molecular Medicine

ISSN: 1528-3658