Fig. 1

Glab inhibits the activation of the cGAS-STING signaling pathway. A Glabridin (Glab) structure. B and C BMDMs were first treated with DMSO or Glab of various concentrations for 1 h, and then stimulated with HT-DNA or Poly(I:C) for 2 h. Whole cell lysate (WCL) was collected and immunoblotted with the indicated antibody. D–F BMDMs were treated with DMSO or Glab of various concentrations for 1 h, and then stimulated with HT-DNA for 4 h. The expression of IFN-β, IL-6 and TNF-α mRNA level were detected by quantitative polymerase chain reaction (qPCR) assay. G–I BMDMs were treated with DMSO or Glab of various concentrations for 1 h and then stimulated with Poly(I:C) for 4 h. The expression of IFN-β, IL-6 and TNF-α mRNA were detected by qPCR assay. Data in D–I are presented as mean ± SEM from three biological replicates. one-way ANOVA and Dunnett’s post hoc test were used to assess the differences of multiple groups, ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 vs. the control, NS, not significant