Fig. 2

Glab is a specific inhibitor of the cGAS-STING signaling pathway. A BMDMs were first treated with DMSO or Glab (20 μM) for 1 h, then stimulated with HT-DNA, Poly(I:C), cGAMP, DMXAA and diABZI to analyze the phosphorylation of IRF3 and the expression of STING in whole cell lysates (WCL) by immunoblotting. B Human PBMCs were first treated with DMSO or Glab (20 μM) for 1 h, and then stimulated with Poly(I:C), cGAMP and diABZI for 2 h. Whole cell lysate was collected and immunoblotted with the indicated antibody. (C–E) BMDMs were first treated with Glab (20 μM) for 1 h and then stimulated with HT-DNA, Poly(I:C), cGAMP, DMXAA and diABZI for 4 h. The expression of IFN-β, IL-6 and TNF-α mRNA were detected by qPCR assay. F–H Human PBMCs were first treated with Glab (20 μM) for 1 h and then stimulated with HT-DNA, Poly(I:C), cGAMP and diABZI for 4 h. The expression of IFN-β, IL-6 and TNF-α mRNA were detected by qPCR assay. Data in (C–H) are expressed as mean ± SEM (n = 3/group, from three biological replicates.). Unpaired student t-test C–H was used to assess differences among groups, ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 vs. the control, NS, not significant