Fig. 4

FGF21 is capable of protecting endothelial cells in vitro by maintaining normal mitochondrial morphology and function. A, B The cell viability test and endothelial cell permeability test for each group. n = 6 per group. C, D Western blots and statistics analysis for ZO-1 and occludin in vitro. n = 6 per group. E, F Representative images of Mitotracker stain and statistics analysis for each group. n = 6 per group. G–J Western blots and statistics analysis for Mfn1, Mfn2 and Drp1 in vitro. n = 6 per group. K, M Representative images of MitoSOX stain and statistics analysis for each group in vitro. n = 6 per group. L, N Representative images of JC-1 and statistics analysis for each group. n = 6 per group. O, P MnSOD and ATP test for each group. n = 6 per group. Scale bar: 10um. ^***P < 0.001 vs Control group and ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 vs OxyHb + Vehicle group by one-way ANOVA (A, B, F, H–J and M–P), ^***P < 0.001 vs Control group and ^&&P < 0.01 vs OxyHb + Vehicle group by two-way ANOVA (D). Values are presented as mean \(\pm\) SD