Fig. 6

FGF21 fails to protect mitochondrial morphology and function in eSIRT6^−/− mice after ICH. A Schematic Figures for the construction of eSIRT6^−/− mice. B Genomic PCR for WT mice, SIRT6^flox/− mice, SIRT6^flox/flox mice and Cre-ERT2 SIRT6^flox/flox mice. C Representative transmission electron microscopy images for mitochondria among SIRT6^flox/flox mice and eSIRT6^−/− mice. Red triangle: representative mitochondria. D, E MnSOD and ATP test for ECs extracted from SIRT6^flox/flox and eSIRT6^−/− mice 72 h after ICH injury. n = 6 per group. Scale bar: 0.25 um. ^**p < 0.01 indicates ICH + Vehicle vs ICH + FGF21 group by two-way ANOVA (D, E). ns: no significant. Values are presented as mean \(\pm\) SD